Barley Barley Crop Icon Brassicas Brassicas Crop Icon Sugar Beet Sugar Beet Carrots Carrots Icon Leeks Leek Icon Maize Maize Icon Oilseed Oilseed Icon Onions Onions Icon Other Cereals Other Cereals Icon O R T Peas And Beans Peas and beans Icon Potatoes Potatoes Icon Salad Crops Salad Crops Icon Soft Fruits Crops Soft Fruits Icon Top Fruits Crops Top Fruits Icon Wheat Crops Wheat Icon Calendar Calendar icon Arrow Next Arrow Previous Close Checkmark

Serenade ASO

Serenade ASO (active substance: Bacillus subtilis strain QST 713) is a broad-spectrum fungicide for the reduction of fruit damage caused by grey mould (Botrytis cinerea) on protected strawberry crops, with EAMUs for numerous other crops.

Go to document store

What is Serenade?

A fungicide for the reduction of fruit damage caused by grey mould (Botrytis cinerea) on protected strawberry crops.

Product Profile

Active substance 1015.1 g/L Bacillus subtilis strain QST 713
Formulation Suspension Concentrate (SC)
Pack Size 10 Litres
Water Volume
Minimum 400 L/ha
Buffer Zone None
MAPP 16139

Important Information

Crops Max. individual dose
Max. number of treatments
Latest time of application
Strawberry (protected) 10.0 L/ha 20 per crop 0 days before harvest


Do not apply using irrigation equipment

Consult processor before using on crops for processing



Why you need Serenade

Get more ways to improve crop quality

It's time to enhance your crop protection. Use Serenade ASO biological fungicide to boost marketable yield in protected strawberries and a wide range of other crops covered by EAMUs.

Serenade ASO principally targets Botrytis losses. However, with a multi-site physical mode of action and the ability to trigger the plant’s own defences, Serenade ASO has been shown to offer broad-spectrum activity against other important fungal and bacterial diseases on the plant and in the soil, as well as resistance management and plant health promotion benefits.


You can apply Serenade ASO as a stand-alone, but for optimum efficacy and resistance management, it is best used in IPM programmes to support conventional fungicides. Moreover, an exemption from residues and a zero-day pre-harvest interval (PHI) mean that it is easy to meet end-of-season customer requirements.


Serenade ASO fits your current practices. It can be used as a tank-mix partner with many crop protection and nutrient products, is approved by the Institute for Marketecology (IMO) for organic use and supported by the Soil Association (SA) and Organic Farmers & Growers (OF&G)*. Storage is straightforward – there are no specific requirements – and Serenade ASO shows excellent safety with a wide range of beneficials.


UK soft fruit growers have successfully integrated Serenade ASO in their fungicide programmes for many years. More recently, it has also become integral to IPM on much of the UK's tomato production for Botrytis and powdery mildew control. 

Serenade ASO is widely used in many areas of leafy salad and baby leaf production - indoor and out, conventional and organic. It's now also a mainstream treatment for carrot cavity spot (Pythium spp.) control and has a strong, growing following in many countries for protecting potato yield and quality where Rhizoctonia is present. 

* Contact your organic certification body before use for current approval status. 


How Serenade works

Foliar application

During fermentation, Serenade ASO's beneficial bacteria produce a range of anti-fungal biological compounds including a group kown as lipopeptides. The lipopeptide profile of this biological chemistry is unique to the specially selected strain of Bacillus in Serenade, QST 713, and is not shared by other B. subtilis or B. amyloliquefasciens strains found in alternative products.

For foliar uses, the lipopeptide component of the biological chemistry is most important. On contact, these lipopeptides disrupt the cell membranes of the pathogen, resulting in physical breakdown of its cells. This mode of action is unique to fungicides based on B. subtilis and B. amylioquefasciens strains and is recognised by the Fungicide Resistance Action Committee (FRAC) as Code 44.


Soil application

Serenade ASO actively colonises roots, forming a protective physical barrier against soil diseases, such as Pythium spp. This barrier grows along with the roots, resulting in extended periods of root protection. As a result, crops can benefit from enhanced plant health and higher marketable yields.


Key characteristics


The different classes of lipopeptides within the biological compounds that Serenade ASO produces act on fungal pathogens in several contact-active ways. Serenade ASO is not systemic within the plant.


This membrane disruption mode of action is especially important when Serenade ASO is used alongside a second fungicide, as it may allow the second fungicide better access to the pathogen.


Once fungal diseases are established in a plant, contact-acting fungicides such as Serenade ASO often have insufficient curative activity to eliminate the pathogen. Preventative applications are therefore vital and application intervals of 7 days or less may be required to prevent fungal re-infection.

Systemic resistance to disease

Serenade ASO has been shown to induce systemic resistance responses in treated plants. In addition to contact control , it primes plants to defend themselves against pathogens. The induced response results in reduced disease and other measurable effects including activation of the salicylic acid (Systemic Acquired Resistance) and gibberellic acid (Induced Systemic Resistance) pathways - observed in both foliar and soil applications.

Major benefits


Because the MoA physically disrupts pathogen membranes, Serenade ASO has been shown to be active against a broad spectrum of diseases. In protected strawberries, Serenade ASO reduces fruit damage caused by Botrytis cinerea, but trials in this and many other crops also show useful activity against diseases such as powdery mildews, Alternaria and Sclerotinia.

Another group of biological compounds produced by Serenade ASO has been demonstrated to have efficacy against bacterial diseases including Clavibacter, Ralstonia, Erwinia, Pseudomonas and Xanthomonas species. Some of these compounds compete for an enzyme that pathogenic bacteria need to build their cell walls.


Serenade ASO is at inherently low risk for the development of resistance. As the major target is the cell membrane, no simple mutation can result in pathogen resistance. In fact, Serenade AO's multiple MoAs are great resistance breakers.

Mutual relationship

The beneficial bacteria in Serenade ASO form a mutual relationship with the plant in the root zone. The root exudates feed the growing bacillus biofilm, which in turn protects and promotes the plant's health through production of its many biological compounds.


Enhanced Root Structure

The colonisation of the roots by Bacillus subtilis not only produces anti-fungal, anti-bacterial and plant health stimulating compounds, but also produces plant growth hormones. These trigger enhanced root development, with visible increases in root laterals and fine root hair development and can speed early-season growth. 


Improves nutrient mobilisation and uptake

Serenade ASO can improve nutrient solubilisation in soil, allowing plants more efficient access to essential nutrients like phosphate and potassium as well as the micronutrients manganese, calcium, boron, zinc and silicon. Improved nutrient availability within the plant following Serenade ASO applications has been seen to result in enhanced crop quality and shelf life of fresh produce.

Roots colonised by Serenade ASO are denser and have more lateral roots and longer root hairs. This increased root surface area gives improved phosphorus uptake, availability of nutrients, water absorption and anchorage - all leading to stronger, healthier plants.



When and how to apply

Serenade ASO is a biofungicide containing Bacillus subtilis QST 713, naturally occurring bacteria that are not genetically modified, for the reduction of fruit damage caused by grey mould (Botrytis cinerea) in protected strawberry crops.

It may be applied as a foliar spray alone or in alternating spray programmes with other registered crop protection products.

Alternating with fungicides of different modes of action, along with the use of cultural control methods, is advisable for resistance management.

For maximum effectiveness, Serenade ASO should be
 applied prior to or in the early stages of disease development. When disease pressure is high, use Serenade ASO in rotation with suitable approved chemicals.

Application rates of Serenade ASO should be
 10 L/ha, applied up to every 7 days or in rotation with a suitable approved chemical.

Serenade ASO should be applied in sufficient water to ensure good crop cover, with a minimum of 400 L/ha.

Programme planning

The diagrams below illustrate how Serenade can be integrated with the use of conventional crop protection chemistry in strawberry disease control programmes based on the following principles;

Begin with the strongest product – Luna Sensation – for a clean start at the critical flowering period and repeat application when under periods of higher disease pressure.

Use Serenade between or alongside applications of conventional chemistry, under periods of lower disease pressure and later when plants are successively flowering and fruiting

Incorporate and alternate conventional chemistry with different / complimentary modes of action


EAMU – Foliar spray protected and outdoor crops

This extension of authorisation (number 0306 of 2015) relates to the use of Serenade ASO as a fungicide for the control of Botrytis spp. in protected and outdoor crops as listed below.

Bulb vegetables
Stem vegetables
Fruiting vegetables
Leafy vegetables
Vegetable brassicas
Root and tuber crops




Ribes hybrids
Outdoor strawberry
Top fruit
Cane fruit
Table grapes
Wine grapes
Canary grass

Crop situations have been defined according to their primary crop groups (please see the CRD crop hierarchy for further details) with the exception of the ‘soft fruit’ group. The ‘soft fruit’ primary group has been divided into the parent group 'cane fruit' and the basic crop or situations of the 'bush/small fruit crops'. The product can only be used on the crops listed in the crop hierarchy under each of the primary crop and parent groups as listed above.

Applications should be made via conventional hydraulic sprayers including air assisted sprayers and hand held sprayers in a minimum of 100 litres water per hectare and a maximum of 1000 L water/ha (to just before run-off). The product should be applied every 7 days or in rotation with a suitable authorised plant protection product.

Max. individual dose (L/ha)
Max. number of treatments 20 per crop
Latest time of application
0 days before harvest

Neither the efficacy nor the phytotoxicity of the product for which this Extension of authorisation has been granted has been assessed and, as such, the user bears the risk in respect of failures concerning its efficacy and phytotoxicity.

EAMU - Drench outdoor crops only

This Extension of Authorisation relates to the use of Serenade ASO applied as a root drench during drilling, planting or transplanting (via hydraulic sprayer or drip feed irrigation lines in 100 to 1000 litres of water depending on method of application) as a preventative treatment for various soil borne fungal infections on a wide range of crops.

Crops Uses


Control of Streptomycetes,
Rhizoctonia and Helminthosporium
Broccoli, Brussels sprout, Cabbage
Calabrese, Cauliflower, Collard, Kale
Control of Pythium spp. and damping off
Bulb onion, Garlic, Leek
Salad onion, Shallot
White rot and damping off
Control of Pythium spp. (cavity spot) and damping off
Control of damping off

Lettuce (excluding lamb's lettuce)

Control of Rhizoctonia and butt rot

Baby leaf production (crops harvested
no later than 8 true leaf stage),
Lettuce, Chard, Spinach, Herbs
(See EAMU for full list)

Damping off
Marrow, Squash, Pumpkin
Courgette, Cucumber
Control of Phytophthora and Pythium spp.

Tomato, Pepper, Aubergine, Chilli

Control of Pythium spp.
Blackberry, Loganberry and rubus hybrid,
Raspberry, Blackcurrant and redcurrant,
Blueberry, Cranberry, Gooseberry
Control of Phytophthora

Neither the efficacy nor the phytotoxicity of the product for which this Extension of authorisation has been granted has been assessed and, as such, the user bears the risk in respect of failures concerning its efficacy and phytotoxicity.

Max. individual dose (L/ha)
Max. number of treatments 1 per crop (per year)
Latest time of application

EAMU – Outdoor cabbage, post-harvest drench

This extension of authorisation relates to the use of Serenade ASO as a fungicide for the control of Botrytis cinerea as a post-harvest drench treatment in outdoor cabbage, before storage.

The treatment solution must not exceed 3% (i.e 30 mls product per litre of spray solution) with a maximum application rate of 20 litres spray solution (600 ml product) per tonne of cabbages.

Application must only take place where any product runoff from the crates/ storage bins and spray equipment washings can be contained (e.g. on a biobed or bunded residue collection area). All dilute pesticide waste must be disposed of safely and legally.

Max. individual
dose (ml/tonne)

Max. number of
treatments (per crop)

Latest time of application

EAMU number

600 as a post-harvest
drench treatment



2825 of 2018

Neither the efficacy nor the phytotoxicity of the product for which this extension of authorisation has been granted has been assessed and, as such, the user bears the risk in respect of failures concerning its efficacy and phytotoxicity.



Talk to us

Contact one of our technical team for more information or advice on Serenade ASO

Your local technical team